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Molecular Biology
The Cobre Biology Core Facility has a fully functional molecular biology lab. Most of the lab is located in IACC 308. Also have had and are planning more workshops and in-services for faculty and students for NDSU and other Universities. Please check out our Workshops link for the next date. The specifics of the lab are below but if you still have questions please feel free to contact us. Check our price list for the fees for this part of the facility.
Available equipment:
- General Incubators
- Shaking Incubator
- Refrigerator and -20°C Freezer
- Water Baths and Heat Blocks
- Agilent 2100 Bioanalyzer
- ABI 7500 Real-Time PCR
- 2 ABI 2720 Thermal Cyclers
- Axon 4000B Genepix Microarray Scanner
- Allegra 25R table-top centrifuge with multiple rotors and adaptors
- Multiple Micro centrifuges
- pH Meters
- Rainin Pipettes (Single and MultiChannel)
- Beckman Coulter DU 530 UV/Vis Spectrometer with Microcell-cuvette
- Gemini EM Microplate Fluorometer (Molecular Devices)
- Gel Electrophoresis Units (Agarose and SDS Page)
- Access to autoclaves, -80°C Freezer, and Sorvall RC-5B Refrigerated Superspeed Centrifuge
Supplies:
Molecular Biology Kits: We stock many kits made by Invitrogen and Qiagen. Below is a list of the ones we regularly have in stock. We would be happy to order different types of kits for you.
Qiagen:
Hispeet Plasmid Midi Kit (DNA)
Qiaprep Spin Miniprep Kit (DNA)
Qiashredder (RNA)
RNeasy Mini and Midi Kits (RNA)
Ni-NTA Spin (Protein)Invitrogen:
Micro-Fasttrack mRNA Isolation Kit
Expressway Plus Expression System
TOPO TA Cloning Kit with C-terminal His Tag
Charges for Luria broth (LB), agar, petri dishes, agarose, real time PCR reagents, cloning enzymes and several restriction enzymes are available as well.
Appointments:
To use this facility for the first time please contact Sheri Dorsam or John Smith for an appointment
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