Due date: March 27, 2008
You will work in groups of two. The list below shows who your teammate is. Below are four data sets for you to analyze using the Mapmaker software. The first set is the one used to demonstrate the software in class. I would suggest you use the dataset to become familiar with the features of the software. You do not need to turn that analysis in. Datasets two, three, and four are of increasing complexity (and therefore of different point value). Dataset four is a complete set from an Arabidopsis mapping project. For that analysis, you are only required to map one linkage group. Here is your linkage group assignment. (I have listed the first three markers for that group, based on the "Group 3 30" command, so you know you have selected the correct one for analysis.)
Group 1
(1 6 9, etc): Fernanado and Shireen; Mu'een and Yuanjie
Group 2 (2 16 26, etc): Guojia and Jerrilyn; Paul and
Noble
Group 3 (3 7 20, etc): Nitin and Rabiya
Group 4 (4 5 13, etc): Junyun and Wei
Group 5 (8 11 31, etc): Nilwala and Hongxia
Group 6 (1 6 9, etc): Aakansha and Vibin
Group 7 (2 16 26 etc): Yueqiang and Santosh
For each data set, you are to:
1. Write a Materials
and Methods section that explains briefly the basic steps you used to
develop your map. (This should be written in manuscript format.)
2. Turn in the final map that shows the linkage order
and distances.
3. State the robustness
of the map, and how it was determined.
4. Provide
evidence from Mapmaker output that supports your map and the statement
regarding its robustness.
Mapmaker Data Files:
- Dataset 1: araba.raw
- Dataset 2: beanbc.raw
- Dataset 3: arab1.raw
- Dataset 4: arab2.raw