PLSC 631 - Intermediate Genetics

Genomic Analysis Quiz

1. Flourescence in situ hybridization has also been called chromosome painting? Suggest a reason why you think this term is appropriate for FISH.

2. Describe the principles behind meiotic recombination mapping.

3. You are collaborating with a cell biologist who has developed four human-rodent hybrid cell lines. Your analysis of the enzyme activities of these four lines revealed the enzyme karros was active in cell lines A and D, enzyme piazza was active in line B, enzyme mondesi was active in line C, and enzyme nomo was active in all cell lines. From the table below determine upon which chromosome these enzymes are located. (The "+" symbol means the chromosome is present in the cell line, and "-" means the chromosome is missing in the line.)

Human Chromosome
Hybrid Colony 1 2 3 4 5 6 7 8
A + - + - + - + -
B + - - + + + - +
C - + + - + + - +
D - + + - + + + +

4. A series of eight STS markers were scored on six YACs. The results are shown in the table below where a "+" means the marker was detected on the YAC, and "-" means the marker was not detected on the YAC. From this information: a. determine the order of the STS markers; and b. order the YACs to form a contig.

STS Marker
YAC A D E F G H X Z
1 - - - + + - - -
2 - - + - - + + +
3 - + - + + - - -
4 - - - + - + - -
5 + - + - - - - +
6 + - - - - - - +