PLSC 631 - Intermediate Genetics
Eukaryotic Chromosome Structure Quiz
1. You have isolated several new temperature senstitive E. coli mutants. You notice that DNA replication is altered when you grow these at the sensitive temperature. From the phenotype give, hypothesis which gene and its function might be non-functional in these mutants.a. Single-stranded DNA molecules appear, but DNA synthesis does not begin.
b. Leading-strand and Okazaki fragments are completed but DNA synthesis is never completed.
c. Ocassionally, an adenine is inserted opposite a cytosine.
2. Are the following relationships between nucleotides true or false?
a. A+T=G+C
b. A+G=C+T
c. G/C=1
d. A=T
3. Discuss how the replication of the lagging strand at the telomere is accomplished in eukaryotes.
4. You are a molecular biologist working with a group of biologists investigating life at the thermal vents in the ocean. Two new prokaryotic organisms are given to you, and the biologists would like to know which has the more complex genome. How would you proceed using the techniques discussed so far in class? What results would lead you to conclude that one genome is more complex than the other?