Isolate #	Light Microscopy		DNA	Type	Sequencing
W Twin 9/21 T-3w Site Location:    West Twin Lake Collection date:  09/21/00 Collection:        tychoplankton SRP:                 0.02045 mg/L NOx:                 0.54414 mg/L Conductivity:     228 ¼S pH:                    7.3		WTwin9-21T-3w 07/23/03 Cultured started 05/27/03 Unidentified coccoid; Large vacuole; red/orange bodies; Probably a eustigmatophye	X	18S Type K
WTwin 8/18 T-5d   Site Location:    West Twin Lake Collection date:  08/18/01 Collection:        tychoplankton SRP:                 0.0731 mg/L NOx:                 0.04299 mg/L Conductivity:     244 ¼S pH:                    7.88		Notes on WTwin 8/18T-5d 02/03/04 culture started 1/23/04  eustigmatophyte	X	18S Type H
WTwin 8/18 T-6d   Site Location:    West Twin Lake Collection date:  08/18/01 Collection:        tychoplankton SRP:                 0.0731 mg/L NOx:                 0.04299 mg/L Conductivity:     244 ¼S pH:                    7.88		Notes on WTwin 8/18 T-6d Eustig 3/9/04 culture started 2/18/04 - grey chamber, 14:10 L:D  WH medium   Cells mostly in clumps on the bottom of the flask.  Spherical cells with many refractile granules, often in motion.  Orange body in many cells, even smallest cells.   The orange body is granular and irregular shaped, not globular as in some other eustigs. The maximum cell size is much greater than other Nannochloropsis isolates, over 10 um.  Pappila not seen. Culture somewhat old.  This isolate seems to grow more slowly than other Nannochloropsis and has a different growth form. Zoospores observed - not Nannochloropsis	X	18S Type G
WTwin 8/18 T-13 Site Location:    West Twin Lake Collection date:  08/18/01 Collection:        tychoplankton SRP:                 0.0731 mg/L NOx:                 0.04299 mg/L Conductivity:     244 ¼S pH:                    7.88
WTwin 8/18 T-15d   Site Location:    West Twin Lake Collection date:  08/18/01 Collection:        tychoplankton SRP:                 0.0731 mg/L NOx:                 0.04299 mg/L Conductivity:     244 ¼S pH:                    7.88			X
Bog D 9/21 T-2d   Site Location:   Bog D Collection date:  09/21/00 Collection:        tychoplankton SRP:                 0.2694 mg/L NOx:                 0.079227 mg/L Conductivity:     595 ¼S pH:                    6.8		Notes on BogD9-21T-2d  DYIV 09/24/03 10/16/03   eustigmatophyte	X	18S Type A