Isolate #	Light Microscopy	General notes-Light microscopy	DNA	Type	Sequencing
Pic 9/21 T-1w   Site Location:    Picnic Pond Collection date:  09/21/00 Collection:        tychoplankton SRP:                 0.19205 mg/L NOx:                 0.033224mg/L Conductivity:     427 μS pH:                   6.71		Notes on Pic9/21T-1w 2/18/04 culture started 1/23/04 - grey chamber, 14:10 L:D   eustigmatophyte	X	18S Type L
Pic 9/21 T-1d   Site Location:    Picnic Pond Collection date:  09/21/00 Collection:        tychoplankton SRP:                 0.19205 mg/L NOx:                 0.033224mg/L Conductivity:     427 μS pH:                    6.71			X	18S Type K
Pic 9/21 T-2d   Site Location:    Picnic Pond Collection date:  09/21/00 Collection:        tychoplankton SRP:                 0.19205 mg/L NOx:                 0.033224mg/L Conductivity:     427 μS pH:                   6.71		Notes on Pic9-21T-2d 9/23/03 culture started 7/30/03, WH  Culture dense Old culture, probably a xanthophyte or eustigmatophyte	X	18S Type R
Pic 6/3 T-4d Site Location:    Picnic Pond Collection date:  06/03/01 Collection:        tychoplankton SRP:                 0.09975 mg/L NOx:                 0.069718 mg/L Conductivity:     280 μS pH:                    7.64
Pic 8/18 T-12w   Site Location:    Picnic Pond Collection date:  08/18/01 Collection:        tychoplankton SRP:                 0.29865 mg/L NOx:                 0.07126 mg/L Conductivity:     369 μS pH:                    7.42		Notes on Itas 8/18 T-12w 11/5/03 culture started 10/9/03 Nearly colorless coccoid.  Spherical.  Probably autosporic, but reproduction not seen	X
Pic 8/18 T-13w   Site Location:    Picnic Pond Collection date:  08/18/01 Collection:        tychoplankton SRP:                 0.29865 mg/L NOx:                 0.07126 mg/L Conductivity:     369 μS pH:                    7.42		Notes on Pic 8/18 T-13w 11/5/03 culture started 10/9/03 Large spherical coccoid, often somewhat ovate or with one side drawn to a point.  Multiple plastids, orange body.  Many highly refractile granules in motion.  Appears to be autosporic.  Eustig	X	18S Type Q
Pic 8/18 T-19w   Site Location:    Picnic Pond Collection date:  08/18/01 Collection:        tychoplankton SRP:                 0.29865 mg/L NOx:                 0.07126 mg/L Conductivity:     369 μS pH:                    7.42		Notes on Pic 8/18 T-19w WH media 12/4/03 culture started 11/20/03 Large spherical eustig with very thin walls. Plastid a thin parietal plate.  Orange body very small or lacking.  Lots of refractile granules, but not in motion. Forming clumps. Reproduction not seen.	X
Pic 8/18 T-15d   Site Location:    Picnic Pond Collection date:  08/18/01 Collection:        tychoplankton SRP:                 0.29865 mg/L NOx:                 0.07126 mg/L Conductivity:     369 μS pH:                    7.42		Notes on Pic8/18T-15d 2/18/04 culture started 1/23/04 - grey chamber, 14:10 L:D   eustigmatophyte; found in clumps	X
Pic 8/18 T-17d  Site Location:    Picnic Pond Collection date:  08/18/01 Collection:        tychoplankton SRP:                 0.29865 mg/L NOx:                 0.07126 mg/L Conductivity:     369 μS pH:                    7.42		Notes on Pic 8-18T-17d 02/03/04 culture started 1/23/04  eustigmatophyte	X
Pic 8/18 T-23d   Site Location:    Picnic Pond Collection date:  08/18/01 Collection:        tychoplankton SRP:                 0.29865 mg/L NOx:                 0.07126 mg/L Conductivity:     369 μS pH:                    7.42		Notes on Pic 8/18 T-23d 2/3/04 culture started 1/23/04, DYIV Spherical Eustig. Culture not dense.	X
Pic 8/18 T-24d   Site Location:    Picnic Pond Collection date:  08/18/01 Collection:        tychoplankton SRP:                 0.29865 mg/L NOx:                 0.07126 mg/L Conductivity:     369 μS pH:                    7.42		Notes on Pic 8/18 T-24d 3/1/04 culture started 2/18/04 - grey chamber, 14:10 L:D   Sometimes in clumps.  The cells in clumps mostly spherical.  Other cells Irregular-somewhat pointed cells, nearly spherical, or somewhat elongate or reniform.  Papilla typcially small or not seen.  Many cells with granular inclusions, but not very mobile.  Larger cells typically with multiple plastids or at least the appearance of multiple plastids. Red or orange spots seen in only a few cells.  Autosporulation not seen and only a very few remnant mother cell walls. This culture is contaminated with an amoeba.	X
Pic 8/18 P-10w   Site Location:    Picnic Pond Collection date:  08/18/01 Collection:        surface SRP:                 0.29865 mg/L NOx:                 0.07126 mg/L Conductivity:     369 μS pH:                    7.42		Notes on Pic 8/18 P-10w 7/17/02 Culture started 6/28/02, WH Large nearly spherical coccoid.  Not autosporic.  Plastid with multiple granuales (starch grains?) and pyrenoid.  Numerous vacuoles.  Occassionally cells divide multiple times to form a short chain. Forms film or flakes in culture.	X
Pic 8/18 P-2d   Site Location:    Picnic Pond Collection date:  08/18/01 Collection:        surface SRP:                 0.29865 mg/L NOx:                 0.07126 mg/L Conductivity:     369 μS pH:                    7.42		Notes on Pic 8/18 P-2d 11/25/02 Culture started 11/14/02, WH Mostly spherical eustigmatophyte.  Red body only seen in some cells.  In many other cells, a similar but uncolored body can be seen. Division not observed, but old walls present.	X
Pic 8/18 P-9d   Site Location:    Picnic Pond Collection date:  08/18/01 Collection:        surface SRP:                 0.29865 mg/L NOx:                 0.07126 mg/L Conductivity:     369 μS pH:                    7.42
Pic 8/18 P-13d   Site Location:    Picnic Pond Collection date:  08/18/01 Collection:        surface SRP:                 0.29865 mg/L NOx:                 0.07126 mg/L Conductivity:     369 μS pH:                    7.42			X