Isolate #	Light Microscopy		DNA	Type	Sequencing
Itas 9/21 S-8w  Site Location:    Lake Itasca Collection date:  09/21/00 Collection:           surface SRP:                    0.0289 mg/L NOx:                  0.047359 mg/L Conductivity:     315 μS pH:                       7.89		Notes on Itas 9/21 S-8w 1/24/01 culture started 12/21/00 Very large coccoid.  Nearly spherical always.  Lots of large inclusions, like egg yolks.  Plastids a twisted band or multiple plastids?  Autospores not observed, but what could be old mother cell walls are present.	X	18S Type M	18S
Itas 9/21 S-11w Site Location:    Lake Itasca Collection date:  09/21/00 Collection:           surface SRP:                    0.0289 mg/L NOx:                  0.047359 mg/L Conductivity:     315 μS pH:                       7.89		Notes on Itas 9/21 S-11w 1/24/01 culture started 12/21/00 Nearly sphaerical.  Autospores not observed, but what could be old mother cell walls are present.	X	18S Type K	18S
Itas 6/3 T-8w Site Location:    Lake Itasca Collection date:  06/03/01 Collection:           tychoplankton SRP:                    0.0146 mg/L NOx:                  0.038878 mg/L Conductivity:     340 μS pH:                       8.39		Itas6/3T-8w 7/27/03 Culture started 5/27/03 - old culture Probably a eustigmatophyte	X
Itas 8/18 M-3d Site Location:    Lake Itasca Collection date:  08/18/01 Collection:        mid-depth, 14ft SRP:                 0.08545 mg/L NOx:                  0.065092 mg/L Conductivity:     294 μS pH:                    8.33		Notes on Itas 8/18 M-3d 6/26/02 Culture started 6/13/02 Large spherical coccoid.  Perforated plastid with buff-colored body.  Some cells have small red bodies, more crystaline than droplets.  May be a eustigmatophyte.	X
Itas 8/18 S-5d Site Location:    Lake Itasca Collection date:  08/18/01 Collection:        surface SRP:                 0.13355 mg/L NOx:                 0.064321 mg/L Conductivity:     295 μS pH:                    8.39		Notes on Itas 8/18 S-5d 7/17/02 Culture started 6/13/02, grown up in WH Nearly spherical to angular cells.  Multiple lenticular plastids without pyrenoids or granules. Probably a eustigmatophyte because of the presence of a yellowish body in the cytoplasm. Reproduction not observed, but probably autosporic.  Old mother cell walls sometimes attached or associated with cells.	X
Itas 8/18 T-7w Site Location:    Lake Itasca Collection date:  08/18/01 Collection:        tychoplankton SRP:                 0.13355 mg/L NOx:                 0.064321 mg/L Conductivity:     295 μS pH:                    8.39			X